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tc20 tm data analyzer software
Tc20 Tm Data Analyzer Software, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/data+analyzer+software/TC20+Data+Analyzer+Software/pmc12869678-446-0-6
Average 99 stars, based on 7 article reviews
Tc20 Tm Data Analyzer Software, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/data+analyzer+software/TC20+Data+Analyzer+Software/pmc12869678-446-0-6
Average 99 stars, based on 7 article reviews
tc20 tm data analyzer software - by Bioz Stars,
2026-10
99/100 stars
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1) Product Images from "Mast cell-specific CysLT2 receptor signaling inhibits cysteinyl leukotriene-dependent mast cell activation and type 2 allergic lung inflammation"
Article Title: Mast cell-specific CysLT2 receptor signaling inhibits cysteinyl leukotriene-dependent mast cell activation and type 2 allergic lung inflammation
Journal: Cell reports
doi: 10.1016/j.celrep.2025.116758
Figure Legend Snippet: (A) Protocol to induce allergic airway inflammation in Cysltr2 flox /Cpa3 cre (MC-specific Cysltr2 KO) mice and littermate controls. Df (1 μg) was administered intranasally on days 0, 4, 7, 11, 14, and 18. Euthanasia was performed 48 h after the last Df challenge. (B) Total/eosinophil cell counts and eosinophil (%) in BAL fluid of Df -treated Cre− (male, n = 8; female, n = 7) and Cre+ (male, n = 6; female, n = 8) on day 20. Total BAL cell numbers were counted by an automated cell counter (Bio-Rad TC20). Eosinophils were defined as CD45-positive/SiglecF-positive/CD11c-negative by flow cytometry. The percentage of eosinophils in the total BAL fluid cell population was calculated by comparing the eosinophil count to the total CD45 + cell count. (C and D) Total lung ILC2/β7+ MC counts and Il5 transcript expression in Df -treated Cre− (male, n = 8; female, n = 7) and Cre+ (male, n = 6; female, n = 8) on day 20. Lung total cell numbers were determined using an automated cell counter (Bio-Rad TC20). (E) BAL fluid levels of PGD 2 and cysLTs (LTC 4 , LTD 4 , and LTE 4 ) in Df -treated Cre− (male, n = 8; female, n = 7) and Cre+ (male, n = 6; female, n = 8) on day 20. Values are mean ± SEM. * p < 0.05, by Mann-Whitney U test. (F–J) Similar analyses as in (A)–(E) were performed using female Cysltr2 flox /Cpa3 cre mice receiving vehicle (PBS) or CysLT 1 R antagonist montelukast (i.p. 30 mg/kg on days 0, 4, 7, 11, 14, and 18). Values are mean ± SEM. Df -treated Cre− (Veh, n = 14; montelukast, n = 11) and Cre+ (Veh, n = 14; montelukast, n = 11) from independent two experiments. * p < 0.05 compared to Cre −/PBS, # p < 0.05, ## p < 0.01 compared to Cre+/PBS, by two-way ANOVA with Sidak’s multiple comparisons test.
Techniques Used: Flow Cytometry, Cell Characterization, Expressing, MANN-WHITNEY
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Cells were then incubated with rabbit anti-SARS-CoV-2 S protein antibody (Sino Biological, 40589-T62, dilution 1:200) on ice for 45 min, and stained with AF647 donkey anti-rabbit IgG antibody (Biolegend, 406414, dilution 1:500) on ice for 45 min. Data were acquired on a Article Title: Mast cell-specific CysLT2 receptor signaling inhibits cysteinyl leukotriene-dependent mast cell activation and type 2 allergic lung inflammation Article Snippet: ID7000 Spectral Cell Analyzer , SONY , https://www.sonybiotechnology.com/instruments/id7000-spectral-cell-analyzer. .. Article Title: 2025 Abstract Information. Article Snippet: Poster Presentations: Biotherapies, Cellular Therapies, and Immunotherapies P-BT-1 | Qualification of a Residual Peracetic Acid Assay for Amnion Patches Undergoing Supercritical CO2 Sterilization I. Pedraza, A. Srinivasan, J. O'Connell, X. Li, A. Trinh, S. Jones 1 BioBridge Global Background/Case Studies: Human amnion patches, derived from placental membranes, are increasingly used in wound healing, ophthalmology, and regenerative medicine due to their regenerative properties.. However, conventional sterilization methods can compromise their biological function.. Supercritical carbon dioxide (scCO2) sterilization has emerged as a gentler alternative that maintains tissue structure and bioactivity. Flow Cytometry:Article Title: Mast cell-specific CysLT2 receptor signaling inhibits cysteinyl leukotriene-dependent mast cell activation and type 2 allergic lung inflammation. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER AC5-LgBit/pEF4 in pEF4 This paper N/A AC6-LgBit/pEF4 in pEF4 This paper N/A AC7-LgBit/pEF4 in pF4A This paper N/A AC9-LgBit/pEF4 in pF4A This paper N/A LgBit-AC2/pEF4 in pEF4 This paper N/A LgBit-AC4/pEF4 in pEF4 This paper N/A LgBit-AC5/pEF4 in pEF4 This paper N/A LgBit-AC6/pEF4 in pEF4 This paper N/A LgBit-AC7/pEF4 in pEF4 This paper N/A LgBit-AC9/pEF4 in pEF4 This paper N/A SmBiT-GNG2(C68S) in pCAGGS Inoue Lab N/A G protein alpha s long (GNAS) cDNA resource center #GNA0SL0000 GNB1 (Gβ1) in pcDNA3.1 Inoue Lab N/A Homo sapiens protein kinase cAMPactivated catalytic subunit beta (PRKACB), var 1 GenScript OHu02335 Homo sapiens protein kinase cAMPactivated catalytic subunit gamma (PRKACG) GenScript OHu31969 Homo sapiens protein kinase cAMPdependent type I regulatory subunit alpha (PRKAR1A), var 1 GenScript OHu16906 Homo sapiens protein kinase cAMPdependent type I regulatory subunit beta (PRKAR1B), var 1 GenScript OHu01423 Homo sapiens protein kinase cAMPdependent type II regulatory subunit beta (PRKAR2B) GenScript OHu03971 SmBiT-PRKACA Control Vector Promega N2015 SmBiT-PRKACB in pFN35K Flexi Vector This paper N/A SmBiT-PRKACG n pFN35K Flexi Vector This paper N/A LgBiT-PRKAR1A in pFN33K Flexi Vector This paper N/A LgBiT-PRKAR1B in pFN33K Flexi Vector This paper N/A LgBiT-PRKAR2A Control Vector Promega N2015 LgBiT-PRKAR2B in pFN33K Flexi Vector This paper N/A beta Arrestin 1 (ARRB1) (NM_004041) Human Tagged ORF Clone ORIGENE RC201279 Arrestin, beta 1 (ARRB1), transcript variant 2 cDNA resource center #ARRB100002 Arrestin, beta 2 (ARRB2), transcript variant 1 cDNA resource center #ARRB200001 Cysltr2-LgBiT in pFC34K Flexi Vector This paper N/A SmBiT-ARRB1 variant1 in pFN35K Vector This paper N/A SmBiT-ARRB1 variant2 in pFN35K Vector This paper N/A SmBiT-ARRB2 variant1 in pFN35K Vector This paper N/A GRK2 in CAGGS Inoue Lab N/A GRK3 in CAGGS Inoue Lab N/A GRK5 in CAGGS Inoue Lab N/A GRK6 in CAGGS Inoue Lab N/A masGRK3ct_(643–688) in pEF4 This paper N/A (Continued on next page) Cell Reports 45, 116758, January 27, 2026 33 .. REAGENT or RESOURCE SOURCE IDENTIFIER KCTD17 (NM_024681) HUman Tagged ORF Colne ORIGENE CAT#RC206070 KCTD17ct in pEF4 This paper N/A Plasmids for NanoBiT association assay (Gβγ/GRKs, Gq/PLCβ1-4) Inoue Lab N/A Plasmids for NanoBiT G protein dissociation assay (Gs, G12, and Gi) Inoue Lab N/A Predesigned shRNA, Human, PRKACA SIGMA TRCN0000233525 Predesigned shRNA, Human, PRKACB SIGMA TRCN0000002002 MISSION® pLKO.1-puro Non- Mammalian shRNA Control Plasmid DNA SIGMA SHC002 MISSION® TRC2 pLKO.5-puro NonMammalian shRNA Control Plasmid DNA SIGMA SHC202 Software and algorithms FlowJo v10.9.0 flow cytometry data analysis BD Biosciences https://www.flowjo.com Prism 9 statistical analysis GraphPad https://www.graphpad.com/features ID7000 Spectral Cell Analyzer SONY https://www.sonybiotechnology.com/ instruments/id7000-spectral-cell-analyzer TC20TM Data Analyzer Software BioRad https://www.bio-rad.com/webroot/web/ html/lsr/products/cell_counting/product_ overlay/global/tc20-data-ana.html AriaMx software Agilent https://www.agilent.com/en/product/real- time-pcr-%28qpcr%29/real-time-pcr%28qpcr%29-instruments/ariamxsoftware-download ImageJ software NIH https://imagej.net/ij/ Prism 9 statistical analysis GraphPad https://www.graphpad.com/features ID7000 Spectral Cell Analyzer SONY https://www.sonybiotechnology.com/ Article Title: Rewilding catalyzes maturation of the humoral immune system Article Snippet: Dead cells were excluded using Invitrogen Fixable Blue Dead Cell Stain Kit (Thermo Fisher Scientific). .. Cell Culture:Article Title: Mast cell-specific CysLT2 receptor signaling inhibits cysteinyl leukotriene-dependent mast cell activation and type 2 allergic lung inflammation. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER AC5-LgBit/pEF4 in pEF4 This paper N/A AC6-LgBit/pEF4 in pEF4 This paper N/A AC7-LgBit/pEF4 in pF4A This paper N/A AC9-LgBit/pEF4 in pF4A This paper N/A LgBit-AC2/pEF4 in pEF4 This paper N/A LgBit-AC4/pEF4 in pEF4 This paper N/A LgBit-AC5/pEF4 in pEF4 This paper N/A LgBit-AC6/pEF4 in pEF4 This paper N/A LgBit-AC7/pEF4 in pEF4 This paper N/A LgBit-AC9/pEF4 in pEF4 This paper N/A SmBiT-GNG2(C68S) in pCAGGS Inoue Lab N/A G protein alpha s long (GNAS) cDNA resource center #GNA0SL0000 GNB1 (Gβ1) in pcDNA3.1 Inoue Lab N/A Homo sapiens protein kinase cAMPactivated catalytic subunit beta (PRKACB), var 1 GenScript OHu02335 Homo sapiens protein kinase cAMPactivated catalytic subunit gamma (PRKACG) GenScript OHu31969 Homo sapiens protein kinase cAMPdependent type I regulatory subunit alpha (PRKAR1A), var 1 GenScript OHu16906 Homo sapiens protein kinase cAMPdependent type I regulatory subunit beta (PRKAR1B), var 1 GenScript OHu01423 Homo sapiens protein kinase cAMPdependent type II regulatory subunit beta (PRKAR2B) GenScript OHu03971 SmBiT-PRKACA Control Vector Promega N2015 SmBiT-PRKACB in pFN35K Flexi Vector This paper N/A SmBiT-PRKACG n pFN35K Flexi Vector This paper N/A LgBiT-PRKAR1A in pFN33K Flexi Vector This paper N/A LgBiT-PRKAR1B in pFN33K Flexi Vector This paper N/A LgBiT-PRKAR2A Control Vector Promega N2015 LgBiT-PRKAR2B in pFN33K Flexi Vector This paper N/A beta Arrestin 1 (ARRB1) (NM_004041) Human Tagged ORF Clone ORIGENE RC201279 Arrestin, beta 1 (ARRB1), transcript variant 2 cDNA resource center #ARRB100002 Arrestin, beta 2 (ARRB2), transcript variant 1 cDNA resource center #ARRB200001 Cysltr2-LgBiT in pFC34K Flexi Vector This paper N/A SmBiT-ARRB1 variant1 in pFN35K Vector This paper N/A SmBiT-ARRB1 variant2 in pFN35K Vector This paper N/A SmBiT-ARRB2 variant1 in pFN35K Vector This paper N/A GRK2 in CAGGS Inoue Lab N/A GRK3 in CAGGS Inoue Lab N/A GRK5 in CAGGS Inoue Lab N/A GRK6 in CAGGS Inoue Lab N/A masGRK3ct_(643–688) in pEF4 This paper N/A (Continued on next page) Cell Reports 45, 116758, January 27, 2026 33 .. REAGENT or RESOURCE SOURCE IDENTIFIER KCTD17 (NM_024681) HUman Tagged ORF Colne ORIGENE CAT#RC206070 KCTD17ct in pEF4 This paper N/A Plasmids for NanoBiT association assay (Gβγ/GRKs, Gq/PLCβ1-4) Inoue Lab N/A Plasmids for NanoBiT G protein dissociation assay (Gs, G12, and Gi) Inoue Lab N/A Predesigned shRNA, Human, PRKACA SIGMA TRCN0000233525 Predesigned shRNA, Human, PRKACB SIGMA TRCN0000002002 MISSION® pLKO.1-puro Non- Mammalian shRNA Control Plasmid DNA SIGMA SHC002 MISSION® TRC2 pLKO.5-puro NonMammalian shRNA Control Plasmid DNA SIGMA SHC202 Software and algorithms FlowJo v10.9.0 flow cytometry data analysis BD Biosciences https://www.flowjo.com Prism 9 statistical analysis GraphPad https://www.graphpad.com/features ID7000 Spectral Cell Analyzer SONY https://www.sonybiotechnology.com/ instruments/id7000-spectral-cell-analyzer TC20TM Data Analyzer Software BioRad https://www.bio-rad.com/webroot/web/ html/lsr/products/cell_counting/product_ overlay/global/tc20-data-ana.html AriaMx software Agilent https://www.agilent.com/en/product/real- time-pcr-%28qpcr%29/real-time-pcr%28qpcr%29-instruments/ariamxsoftware-download ImageJ software NIH https://imagej.net/ij/ Prism 9 statistical analysis GraphPad https://www.graphpad.com/features ID7000 Spectral Cell Analyzer SONY https://www.sonybiotechnology.com/ Adhesive:Article Title: Mast cell-specific CysLT2 receptor signaling inhibits cysteinyl leukotriene-dependent mast cell activation and type 2 allergic lung inflammation. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER AC5-LgBit/pEF4 in pEF4 This paper N/A AC6-LgBit/pEF4 in pEF4 This paper N/A AC7-LgBit/pEF4 in pF4A This paper N/A AC9-LgBit/pEF4 in pF4A This paper N/A LgBit-AC2/pEF4 in pEF4 This paper N/A LgBit-AC4/pEF4 in pEF4 This paper N/A LgBit-AC5/pEF4 in pEF4 This paper N/A LgBit-AC6/pEF4 in pEF4 This paper N/A LgBit-AC7/pEF4 in pEF4 This paper N/A LgBit-AC9/pEF4 in pEF4 This paper N/A SmBiT-GNG2(C68S) in pCAGGS Inoue Lab N/A G protein alpha s long (GNAS) cDNA resource center #GNA0SL0000 GNB1 (Gβ1) in pcDNA3.1 Inoue Lab N/A Homo sapiens protein kinase cAMPactivated catalytic subunit beta (PRKACB), var 1 GenScript OHu02335 Homo sapiens protein kinase cAMPactivated catalytic subunit gamma (PRKACG) GenScript OHu31969 Homo sapiens protein kinase cAMPdependent type I regulatory subunit alpha (PRKAR1A), var 1 GenScript OHu16906 Homo sapiens protein kinase cAMPdependent type I regulatory subunit beta (PRKAR1B), var 1 GenScript OHu01423 Homo sapiens protein kinase cAMPdependent type II regulatory subunit beta (PRKAR2B) GenScript OHu03971 SmBiT-PRKACA Control Vector Promega N2015 SmBiT-PRKACB in pFN35K Flexi Vector This paper N/A SmBiT-PRKACG n pFN35K Flexi Vector This paper N/A LgBiT-PRKAR1A in pFN33K Flexi Vector This paper N/A LgBiT-PRKAR1B in pFN33K Flexi Vector This paper N/A LgBiT-PRKAR2A Control Vector Promega N2015 LgBiT-PRKAR2B in pFN33K Flexi Vector This paper N/A beta Arrestin 1 (ARRB1) (NM_004041) Human Tagged ORF Clone ORIGENE RC201279 Arrestin, beta 1 (ARRB1), transcript variant 2 cDNA resource center #ARRB100002 Arrestin, beta 2 (ARRB2), transcript variant 1 cDNA resource center #ARRB200001 Cysltr2-LgBiT in pFC34K Flexi Vector This paper N/A SmBiT-ARRB1 variant1 in pFN35K Vector This paper N/A SmBiT-ARRB1 variant2 in pFN35K Vector This paper N/A SmBiT-ARRB2 variant1 in pFN35K Vector This paper N/A GRK2 in CAGGS Inoue Lab N/A GRK3 in CAGGS Inoue Lab N/A GRK5 in CAGGS Inoue Lab N/A GRK6 in CAGGS Inoue Lab N/A masGRK3ct_(643–688) in pEF4 This paper N/A (Continued on next page) Cell Reports 45, 116758, January 27, 2026 33 .. REAGENT or RESOURCE SOURCE IDENTIFIER KCTD17 (NM_024681) HUman Tagged ORF Colne ORIGENE CAT#RC206070 KCTD17ct in pEF4 This paper N/A Plasmids for NanoBiT association assay (Gβγ/GRKs, Gq/PLCβ1-4) Inoue Lab N/A Plasmids for NanoBiT G protein dissociation assay (Gs, G12, and Gi) Inoue Lab N/A Predesigned shRNA, Human, PRKACA SIGMA TRCN0000233525 Predesigned shRNA, Human, PRKACB SIGMA TRCN0000002002 MISSION® pLKO.1-puro Non- Mammalian shRNA Control Plasmid DNA SIGMA SHC002 MISSION® TRC2 pLKO.5-puro NonMammalian shRNA Control Plasmid DNA SIGMA SHC202 Software and algorithms FlowJo v10.9.0 flow cytometry data analysis BD Biosciences https://www.flowjo.com Prism 9 statistical analysis GraphPad https://www.graphpad.com/features ID7000 Spectral Cell Analyzer SONY https://www.sonybiotechnology.com/ instruments/id7000-spectral-cell-analyzer TC20TM Data Analyzer Software BioRad https://www.bio-rad.com/webroot/web/ html/lsr/products/cell_counting/product_ overlay/global/tc20-data-ana.html AriaMx software Agilent https://www.agilent.com/en/product/real- time-pcr-%28qpcr%29/real-time-pcr%28qpcr%29-instruments/ariamxsoftware-download ImageJ software NIH https://imagej.net/ij/ Prism 9 statistical analysis GraphPad https://www.graphpad.com/features ID7000 Spectral Cell Analyzer SONY https://www.sonybiotechnology.com/ Incubation:Article Title: Targeting SARS-CoV-2 viral-immune interaction for COVID-19 therapy Article Snippet: .. Cells were then incubated with rabbit anti-SARS-CoV-2 S protein antibody (Sino Biological, 40589-T62, dilution 1:200) on ice for 45 min, and stained with AF647 donkey anti-rabbit IgG antibody (Biolegend, 406414, dilution 1:500) on ice for 45 min. Data were acquired on a Staining:Article Title: Targeting SARS-CoV-2 viral-immune interaction for COVID-19 therapy Article Snippet: .. Cells were then incubated with rabbit anti-SARS-CoV-2 S protein antibody (Sino Biological, 40589-T62, dilution 1:200) on ice for 45 min, and stained with AF647 donkey anti-rabbit IgG antibody (Biolegend, 406414, dilution 1:500) on ice for 45 min. Data were acquired on a |